The Monoclonal Antibodies aS - HCL 1 ( aLeu - 14 ) and aS - HCL 3 ( aLeu - M 5 ) Allow the Diagnosis of Hairy Cell Leukemia
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چکیده
To define cell surface antigens associated with hairy cell leukemia (HCL). and to gain better insight into the origin of this disease. we developed monoclonal antibodies against spleen cells of a patient with this disease. Although none of these antibodies alone proved specific for the leukemic cells. two of them. designated aS-HCL 1 (aLeu-14) and aS-HCL 3 (aLeu-M5) were found to be valuable in the diagnosis of HCL when used in combination. aS-HCL 1 recognizes an antigen associated with >95% of B cells in the peripheral blood. Biochemical analysis identified this antigen as a single polypeptide chain with a molecular weight of 1 50.000 daltons (1 50 kilodaltons). aS-HCL 1
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